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A) Yeast two-hybrid screen performed with FEZ1 (221–392) as a bait using a human fetal brain <t>cDNA</t> library retrieved 16 proteins that were confirmed to interact all with both FEZ1(221–392) and FEZ2 (207–353) in the one-to-one assay. B) Yeast two-hybrid screen performed with FEZ2 (207–353) as a bait using human fetal brain and bone <t>marrow</t> <t>cDNA</t> libraries retrieved 59 proteins as preys. In the one-to-one confirmation, 19 of these proteins interacted only with FEZ2 (207–353) protein, 40 with both FEZ2 (207–353) and FEZ1 (221–392) and 36 interacted with all FEZ2 (207–353), FEZ1 (221–392) and UNC-76 (242–378).
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A) Yeast two-hybrid screen performed with FEZ1 (221–392) as a bait using a human fetal brain <t>cDNA</t> library retrieved 16 proteins that were confirmed to interact all with both FEZ1(221–392) and FEZ2 (207–353) in the one-to-one assay. B) Yeast two-hybrid screen performed with FEZ2 (207–353) as a bait using human fetal brain and bone <t>marrow</t> <t>cDNA</t> libraries retrieved 59 proteins as preys. In the one-to-one confirmation, 19 of these proteins interacted only with FEZ2 (207–353) protein, 40 with both FEZ2 (207–353) and FEZ1 (221–392) and 36 interacted with all FEZ2 (207–353), FEZ1 (221–392) and UNC-76 (242–378).
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A) Yeast two-hybrid screen performed with FEZ1 (221–392) as a bait using a human fetal brain <t>cDNA</t> library retrieved 16 proteins that were confirmed to interact all with both FEZ1(221–392) and FEZ2 (207–353) in the one-to-one assay. B) Yeast two-hybrid screen performed with FEZ2 (207–353) as a bait using human fetal brain and bone <t>marrow</t> <t>cDNA</t> libraries retrieved 59 proteins as preys. In the one-to-one confirmation, 19 of these proteins interacted only with FEZ2 (207–353) protein, 40 with both FEZ2 (207–353) and FEZ1 (221–392) and 36 interacted with all FEZ2 (207–353), FEZ1 (221–392) and UNC-76 (242–378).
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A) Yeast two-hybrid screen performed with FEZ1 (221–392) as a bait using a human fetal brain cDNA library retrieved 16 proteins that were confirmed to interact all with both FEZ1(221–392) and FEZ2 (207–353) in the one-to-one assay. B) Yeast two-hybrid screen performed with FEZ2 (207–353) as a bait using human fetal brain and bone marrow cDNA libraries retrieved 59 proteins as preys. In the one-to-one confirmation, 19 of these proteins interacted only with FEZ2 (207–353) protein, 40 with both FEZ2 (207–353) and FEZ1 (221–392) and 36 interacted with all FEZ2 (207–353), FEZ1 (221–392) and UNC-76 (242–378).

Journal: PLoS ONE

Article Title: FEZ2 Has Acquired Additional Protein Interaction Partners Relative to FEZ1: Functional and Evolutionary Implications

doi: 10.1371/journal.pone.0017426

Figure Lengend Snippet: A) Yeast two-hybrid screen performed with FEZ1 (221–392) as a bait using a human fetal brain cDNA library retrieved 16 proteins that were confirmed to interact all with both FEZ1(221–392) and FEZ2 (207–353) in the one-to-one assay. B) Yeast two-hybrid screen performed with FEZ2 (207–353) as a bait using human fetal brain and bone marrow cDNA libraries retrieved 59 proteins as preys. In the one-to-one confirmation, 19 of these proteins interacted only with FEZ2 (207–353) protein, 40 with both FEZ2 (207–353) and FEZ1 (221–392) and 36 interacted with all FEZ2 (207–353), FEZ1 (221–392) and UNC-76 (242–378).

Article Snippet: In this work, yeast two-hybrid screens of human fetal brain and bone marrow cDNA libraries (Clontech) were performed as described previously by using the yeast strain L40 (trp1-901, his3Δ200, leu2-3, ade2 LYS2::(lexAop)4-HIS3 URA3::(lexAop)8-lac GAL4) and human FEZ2 (207–353) as a bait in fusion with the LexA protein as encoded in the recombinant vector pBTM116 .

Techniques: Two Hybrid Screening, cDNA Library Assay